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. 2020 Jun 4;11:1045. doi: 10.3389/fimmu.2020.01045

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Copyright © 2020 Cha, Xie, Jin, Feng, Xie, Xie, Yang, Qi, Qiu, Wu, Yin, Mu and Huang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Surface molecule expression on pulmonary γδ T cells. Single lung cell suspensions were separated from naïve and infected mice, and cells were stained with monoclonal antibodies against mice: CD3, γδTCR, CD4, CD8, Vγ2, CD25, CD69, CD127, CD62L, MHC II, CD80, PDL1, PDL2, CXCR3, CXCR4, CXCR6, and CX3CR1. (A) Representative FACS analysis of three independent experiments is shown. (B) Percentages of surface molecules calculated from FACS analysis, a representative experiment from five independent experiments was shown. The error bars are SD, **P < 0.01, *P < 0.05.