Figure 3.

Cytokines released by pulmonary γδ T cells. C57BL/6 mice were infected with 40 ± 5 S. japonicum cercariae per mouse, and 5–6 weeks after the infection, mice were euthanized. Single cell suspensions of lung cells were stimulated with PMA and ionomycin. Cytokine (IL-4, IFN-γ, IL-10, IL-5, IL-17, IL-21, IL-2, GM-CSF, IL-1, and IL-6) expression was detected in CD3⁺γδTCR⁺ cells by FACS analysis. (A) A representative of four independent experiments with 5–6 mice per group is shown. (B) Average expression of different cytokines on CD3⁺γδTCR⁺ cells was calculated by FACS data. The error bars are SD, **P < 0.01, *P < 0.05.