Figure 5.

The effect of γδ T cells on T cells. C57BL/6 and Vδ^−/− mice were infected with 40 ± 5 S. japonicum cercariae per mouse or not, and mice were euthanized after 5–6 weeks of infection. Single lung cell suspensions were separated. (A–D) Percentages and absolute numbers of CD3⁺ T cells, CD3⁺CD4⁺ and CD3⁺CD4⁻ cell population were counted. (E) Activation of CD3⁺ T cells, CD3⁺CD4⁺ T cells and CD3⁺CD4⁻ cells in naïve and infected WT and Vδ^−/− mice. (F) Expression of IL-4, IFN-γ, IL-17, and IL-21 in CD3⁺ T cells, CD3⁺CD4⁺ T cells and CD3⁺CD4⁻ cells in each groups of mice. (G) Secretion of IL-4, IFN-γ, and IL-17 in the supernatant of lung cells cultured under different stimulants. Single lung cell suspensions of each group of mice were prepared and cultured in vitro with anti-CD3 + anti-CD28, SEA + anti-CD28, and SWA + anti-CD28, respectively. The supernatants were collected after 72 h. The secretion of IFN-γ and IL-4 was detected by ELISA. Data represent the average of three independent experiments with 5–6 mice per group. The error bars are SD, **P < 0.01, *P < 0.05.