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. 2020 Jun 4;11:1045. doi: 10.3389/fimmu.2020.01045

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Copyright © 2020 Cha, Xie, Jin, Feng, Xie, Xie, Yang, Qi, Qiu, Wu, Yin, Mu and Huang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of γδ T cells on B cells. C57BL/6 mice and Vδ^−/− mice were infected with 40 ± 5 S. japonicum cercariae per mouse. Five to six weeks after infection, mice were euthanized. Single lung cell suspensions were separated. (A–D) Percentage and number of B cells in the lung of naïve and infected WT and Vδ^−/− mice were detected. (E–G) Expression of CD69 and CD80 on B cells in naïve and infected WT and Vδ^−/− mice. Blood were collected, and serum was diluted in different proportion (1,000, 10,000, and 100,000 folds). The levels of IgG in the serum of four groups were detected by ELISA. (H) Soluble egg antigen (SEA)-specific IgG is shown. (I) Soluble worm antigen (SWA)-specific IgG is shown. Data represent the average of three independent experiments with 5–6 mice per group. The error bars are SD, **P < 0.01, *P < 0.05.