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. 2020 Jun 10;10:9393. doi: 10.1038/s41598-020-66229-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Generation of dog bladder cancer (BC) 2.5D organoids. Schematic experimental design of a procedure for isolation of 2.5D organoid cells from 3D organoids and the analysis overview (A). Representative bright-field images of the process of generation of 2.5D organoids from 3D ones and serially passaged cells (B). Scale bar: 500 μm. Representative images and enlarged ones (Scale bar: 200 μm) for three different strains of 2.5D organoid cells and their parental 3D organoids (C). Comparison of cell attachment and proliferation between the 2.5D organoid media and 2D cell line media (D). Representative images at passage 0 and 1 and enlarged ones at passage 1 were shown. Scale bar: 500 μm.