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. 2020 Jun 10;10:9393. doi: 10.1038/s41598-020-66229-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Tumorigenesis induced by BC 2.5D organoids. The trypsinized 2.5D cells (1×10⁶) were subcutaneously injected into the back of NSG mice (n = 4). Six weeks later, the formed tumors were isolated and sectioned for H&E and immunofluorescence staining. Representative image of the formed tumors and their sizes (A). Representative images of H&E staining of the tumor tissue sections. The enlarged image is shown on the right. Scale bar: 500 and 100 μm (B). Characterization of the cellular components of the formed tumors. Expression of CK7, CK20, and UPK3A in the tumor tissues (C). Representative photomicrographs were shown. Scale bar: 50 μm.