Table 1.

The difference of media components between 2.5D and 3D organoid culture.

Supplement	2.5D organoid	3D organoid
Wnt, Noggin and R-Spondin	−	+
FBS	+	−
EGF	+	+
GlutaMax	+	+
N-Acetyl-l-cysteine	+	+
Nicotinamide	+	+
A83-01	+	+
HEPES	+	+
Antibiotics	+	+