Table 2.
Summary of the relevant details of three landmark studies based on gene expression analysis of purified classical, intermediate and nonclassical monocyte subsets in health.
| Cros et al. (25) | Wong et al. (26) | Zawada et al. (27) | |
|---|---|---|---|
| Gene expression technique | Microarray | Microarray | SuperSAGE |
| GEO |
E-MEXP-2544 E-MEXP 2545 |
GSE3081 | GSE25931 |
| Most closely related populations | Nonclassical & Intermediate | Classical & Intermediate | Classical & Intermediate |
| Demonstrated functional correlations | |||
| Subset | Cros et al. (25) | Wong et al. (26) | Zawada et al. (27) |
| Classical | Cytokine Production: Highest production IL-8, IL-10, CCL2, CCL3 after LPS stimulation. Also produce IL-6 Phagocytosis of latex beads Produced high levels of ROS |
Receptor Expression: Highest expression of CD54, CCR1, CCR2, CXCR1, CXCR2, CXCR4, CD11B, CD33, CD52L, CD1d, CD9, CD99, CLEC4D, CLEC5A, IL13Ra1 Cytokine Production: Produce GM-CSF, IL10, CCL2 after LPS stimulation |
Receptor Expression: Highest levels of CD91, CD64, CD11B, CD35 Lowest ROS production Induction of T-cell proliferation |
| Intermediate | Cytokine Production: Highest production TNF-α, IL-1ß, and IL-6 after LPS stimulation, also produced Phagocytosis of latex beads Did not produce ROS |
Receptor Expression: Highest expression of CD40, CD80, HLA-ABC, HLA-DR, CD32, CCR5, CD54, CD163, CLEC10a, GFRa2 Cytokine Production: Intermediate or lowest level production of all cytokines studied |
Receptor Expression: Highest levels of CD74, HLA-DR, CD202B, CD105 Highest ROS production Most potent induction of T-cell proliferation in vitro Form cellular clusters in an in vitro angiogenesis assay |
| Nonclassical | Cytokine Production: IL-1R antagonist production after LPS stimulation TNF-α and IL-1ß production after viral stimulation Did not phagocytose latex beads Did not produce ROS Patrolling behavior on endothelial layers |
Receptor Expression: Highest expression of CXC3CR1, CD115, CD97, CD123, CD 294, P2RX1, Siglec10 Cytokine Production: Highest production of TNF-α and IL-1ß |
Receptor Expression: Highest levels of CD31, CD43, CD11a, CD47 Intermediate ROS production Induction of T- cell Proliferation in-vitro |