Figure 5.

Properties of CoChR-Mediated Light Responses following BC Targeting in Multielectrode Recordings

(A) Representative recordings of CoChR-mediated spiking activities exhibiting ON and OFF light responses in the presence of L-AP4 (20 μM) and ACET (2 μM) (top panel). The OFF response was blocked by the addition of strychnine (5 μM; middle panel). The ON response was further blocked by the addition of a mixture of CNQX (50 μM), D-AP5 (20 μM), DHβE (20 μM), and bicuculline (50 μM; bottom panel). (B) Representative recordings of CoChR-mediated spiking activities showing ON light responses in the presence of L-AP4 and ACET. The ON response was mostly blocked by the mixture of antagonists (lower panel). The spiking activities were elicited by 1 s light pulses at 480 nm with the light intensity of 2.4 × 10¹⁵ photons/cm²s. In each panel, a raster plot of 10 consecutive recordings, and an averaged spike rate histogram are shown in the top and bottom panels, respectively. The recordings were made from TKO mice.