Figure 6.

Comparison of the CoChR-Mediated Pupillary Constriction in TKO Mice between BC Targeting and RGC Expression

(A and B) Representative images of TKO mice expressing CoChR after BC targeting and RGC expression in response to light stimulation. Marked pupil constriction was observed in mice with RGC expression (A), but not in mice with BC targeting (B). (C) The average percentage of the normalized pupil area in response to light stimulation with BC targeting (78% ± 5.9%; mean ± SD; n = 17) is significantly different from RGC expression (58% ± 20%; n = 15; one-way ANOVA, p < 0.001) but is not significantly different from the untreated control mice (84% ± 3.3%; n = 11). Light intensity: 3 × 10¹⁵ photons/cm²s with blue LED illumination (470 nm).