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. 2020 Jun 8;6(6):e04114. doi: 10.1016/j.heliyon.2020.e04114

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© 2020 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Characterization of GNP. (A) Hydrodynamic sizes of bare Au-GNP and antibody conjugated GNP-2E10 were assessed through dynamic light scattering by using Zetasizer. Inset shows the stable GNP-2E10 that has been optimized with suitable antibody concentration and pH of dispersion medium. The developed GNP-2E10 remained stable in 10 % (w/v) NaOH solution. Prior to upscaled production of Au-GNP-2E10, optimal (B) antibody concentration and (C) pH of dispersion medium essential for production stable conjugate were evaluated via UV-visible spectrophotometry and salt aggregation assay respectively.