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. 2020 Jun 8;6(6):e04114. doi: 10.1016/j.heliyon.2020.e04114

Figure 7.

Figure 7

(A) Comparisons of four parametric logistic (4PL) curves derived from LFIA of two different antibody conjugate combinations: (1) biotin-3H3 antibody + GNP-2E10 antibody and (2) biotin-2E10 antibody + GNP-3H3 antibody. Respective images of developed LFIA test strips were depicted in Figure 6A (B) Detection sensitivity and specificity of oxLDL/β2GPI LFIA and oxLDL/β2GPI ELISA were compared by using Cu2+-oxLDL/β2GPI as test sample. ∗Units (y-axis) for ELISA-based system were plotted in accordance to absorbance recorded at 450 nm (reference wavelength at 600 nm) while units (y-axis) for LFIA-based system were arbitrary units of relative intensities derived from ImageJ analysis. (C) OxLDL/β2GPI contents in serum samples were evaluated with oxLDL/β2GPI ELISA- and LFIA-based assay. The correlation plot showed significant correlation (R2 value >0.826) between ELISA- and LFIA-based assay. Respective images of developed LFIA test strips were depicted in Figure 6C.