Figure 3.

Effects of andrographolide on cell survival and ROS reduction. (A) The effect of the pre-treatment of andrographolide on cell viability. 10 μM of andrographolide was used to treat the cells prior to oxidative stress induction with 1 mM H₂O₂ for 15 min. (B) The same conditions as (A) but for the detection of ROS production. The experiments were performed for at least 3 independent assays. Results are presented as mean ± SD. ∗p < 0.05, ∗∗p < 0.01,∗∗∗p < 0.001 and ∗∗∗∗p < 0.0001 vs control.