Figure 6.

Dolichol Content Is Markedly Decreased in Dhdds^flx/flx iCre⁺ Retinas, Relative to Age-Matched Controls

(A) LC-MS analysis of dolichols extracted from Dhdds^flx/flx iCre⁺ and control retinas demonstrated ∼50% decrease in the total dolichol content of Dhdds^flx/flx iCre⁺ retinas (n = 4), when compared with controls (n = 3) (upper panel, A). However, there was no selectivity as a function of dolichol chain lengths: the Dol-18:Dol-19 ratio [C90:C95] in Dhdds^flx/flx iCre⁺ retinas was comparable to that of age-matched controls (lower panel, A).

(B) Mass spectra of Dol-18 and Dol-19 species from Dhdds^flx/flx iCre⁻ (control) and Dhdds^flx/flx iCre⁺ retinas. Note that signals of ammoniated [M + NH₄]⁺ and sodiated [M + Na]⁺ forms of respective Dol isotopologues are clearly visible. Each sample analyzed was prepared by pooling five retinas from the respective genotype group. ∗p < 0.05; Welch's (unpaired) t test.