Fig. 2.

NO₂-OA inhibits mLDL incorporation by macrophages. A. Cholesterol and cholesteryl ester levels were reduced in RAW264.7 macrophages co-incubated with NO₂-OA (5 μM) and mLDL for 24 h as assessed by LC-MS-MS. Analyses were performed using deuterated cholesterol and cholesteryl palmitate as internal standards. B and C. Immunofluorescence images and quantification of fluorescence from RAW264.7 cells incubated with NO₂-OA (5 μM) and SSO (50 μM) for 15 min follow by treatment with DiI-labeled oxLDL (50 μg/uL) for 2 h at 4 °C and then washed and incubated at 37 °C for 30 min. Quantification of fluorescence was performed on at least 20 cells per condition. D. NO₂-OA but not OA preincubation (15 min) with recombinant CD36 protein inhibits binding of DiI-labeled oxLDL (50 μg/μL). Binding was assessed after incubation for 2 h at 37 °C. The data is obtained from a representative experiment selected from at least 3 independent experiments. Mean ± SEM are represented in graphs (*p < 0.05, **p < 0.01, ***p < 0.001).