Fig. 3.

NO₂-OA binds reversibly and covalently to CD36. A. RAW264.7 macrophages were incubated with B-NO₂-OA for 15 min at 4 °C to limit receptor endocytosis. Immunoprecipitation of CD36 was blotted for B-NO₂-OA using HRP-Streptavidin. B. Recombinant CD36 was incubated with different mole ratios of B-NO₂OA and the complexes B-NO₂-OA-rCD36 revealed by SDS-PAGE using HRP-Streptavidin. C. Competition of B-NO₂-OA with NO₂-OA or OA for rCD36 binding. D. GSH inhibits B-NO₂OA interaction with rCD36. E. SSO displacement of B-NO₂-OA from B-NO₂-OA-rCD36 adduct. The data is from a representative experiment selected from at least 3 independent experiments, n = 3 for each determination showing mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.