Fig. 5.

NO₂-OA regulates cholesterol metabolism. A. RAW264.7 macrophages loaded with DiI-oxLDL (100 μg/ml/24 h) were incubated with vehicle, NO₂-OA or OA during 24 h. Images were obtained in a Leica DMi8 microscope. At least 20 cells per condition were randomly analyzed. The data is from a representative experiment selected from at least 3 independent experiments. B. ABCG1 and ABCA1 mRNA expression in RAW264.7 macrophages treated with 5 μM of NO₂-OA during 2, 4 and 8 h. At least 3 independent experiments were carried out. C. Cholesterol efflux in RAW 264.7 macrophages loaded with oxLDL 100 μg/ml/24 h. Macrophages were treated with vehicle or NO₂-OA (5 μM), cholesterol release was evaluated in the cell culture supernatant by MS. The graph shows the data obtained from at least 3 independent experiments. D. RAW 264.7 macrophages loaded with oxLDL 100 μg/ml for 24 h. Then cells were treated with vehicle or NO₂-OA (5 μM) for 8 h in presence or absence of AMPK inhibitor CompC (3,3 μM). Macrophages were harvested in lysis buffer and samples analyzed by Western blot. The data is from a representative experiment selected from at least 3 independent experiments. Each quantification shows mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.