Evaluation of ‘super-resistant’ fly lines fitness. (a,b): one-way ANOVA of pupation after 7–8 days in strains bearing both resistance alleles for the ‘beetle’ (a) and ‘mosquito’ (b) allele combinations compared to controls, indicating an extended developmental time when both alleles are present. All p-values are shown in the electronic supplementary material, table S4. (c,d): one-way ANOVA of total oviposition in strains bearing both resistance alleles for the ‘beetle’ (c) and ‘mosquito’ (d) allele combinations. Oviposition is significantly reduced in paraV1016G; HR-GAL4>UAS-CYP9J28(2N) flies against all controls, indicating reduced overall fecundity. All p-values are shown in the electronic supplementary material, table S5. For the full life table parameters dataset, see the electronic supplementary material, dataset 1.