Figure 3.

Restricted expression of MICA glycoproteins in MICAgen mice. (A,B) MICA glycoproteins are barely detectable in organs of MICAgen mice. Cryosections of (A) lymph nodes, thymi, small intestines, and colons and (B) skin (ear) of MICAgen mice (middle panels), and for control, of nontgLM (left) and of H2-K^b-MICA mice (right), were stained with biotinylated anti-MICA/B mAb BAMO1 plus SA-BV421 (red) and counterstained with SYTOX Green (blue) for nuclear staining. Exposure times for SA-BV421 were (A) 80 ms and (B) 380 ms, respectively. Results are representative for three MICAgen mice and two H2-K^b-MICA mice analyzed.