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. Author manuscript; available in PMC: 2021 May 28.
Published in final edited form as: Biochem Biophys Res Commun. 2020 Mar 28;526(2):466–471. doi: 10.1016/j.bbrc.2020.03.100

Fig. 1.

Fig. 1.

(A) Like P4, all the selected progestins compete with [3H]-Ald for binding to the human MR. COS-1 cells were transiently transfected with 11.25 μg of the human MR expression vector and incubated for 16 hours with 0.2 nM [3H]-Ald in the absence or presence of increasing concentrations of unlabelled ligands. Counts per minute (cpm) were normalized to the protein concentration (mg/ml). Total specific binding of [3H]-Ald only was expressed as 100% and the binding of unlabelled competitors expressed as a percentage relative to this. (B) Log Kd/Ki values of the ligands for the MR are plotted.