Skip to main content
. Author manuscript; available in PMC: 2021 May 28.
Published in final edited form as: Biochem Biophys Res Commun. 2020 Mar 28;526(2):466–471. doi: 10.1016/j.bbrc.2020.03.100

Fig. 2.

Fig. 2.

Most progestins display differential antagonist potencies for transactivation via the MR, while DRSP and P4 are weak partial MR agonists for transactivation. COS-1 cells, transiently transfected with 1 μg of the human MR expression vector and 10 μg of the pTAT-2xPRE-E1b-luciferase reporter plasmid, were treated with increasing concentrations of Ald, P4 or progestins in the absence (A) or (C) presence of 1 nM Ald (set as 100%) for 24 hours. (B) Induction by P4 and the progestins at 10 μM (from A) is shown as a percentage relative to 10 μM Ald expressed as 100%. Luciferase activity was measured in relative light units and normalized to the protein concentration. (D) Maximal responses and (E) log EC50 values of the ligands for the MR (from C) were plotted.