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. Author manuscript; available in PMC: 2021 May 28.
Published in final edited form as: Biochem Biophys Res Commun. 2020 Mar 28;526(2):466–471. doi: 10.1016/j.bbrc.2020.03.100

Fig. 3.

Fig. 3.

Most progestins display indistinguishable agonist activity for transrepression via the MR on the synthetic NFκB, but not the AP-1, promoter. COS-1 cells transiently transfected with 1.35 μg of the human MR expression vector and 2.7 μg of the (A) 7xAP-1-luciferase or (C) 5xNFκB-luciferase reporter plasmid, were treated with EtOH and 10 ng/ml PMA in the absence (set as 100%) or presence of increasing concentrations of ligands for 24 hours. Luciferase activity was measured and normalized as in Fig. 2. Treatment with 10 ng/ml PMA resulted in a ~14-fold and ~32-fold induction, respectively (Fig. 3A and 3C inserts). (B and D) Log EC50 values of the ligands for the MR were plotted.