Figure 1.

Anti-adipogenic effects of Cornus officinalis (CO) and Ribes fasciculatum (RF) on mRNA expression of adipogenic markers in 3T3-L1 cells. Cells were induced by 3-isobutyl-1-methylxanthine, dexamethasone and insulin multiple daily injections and co-incubated with CO (A) and RF (B) at 2, 10, or 50 μg/mL during lipid accumulation. mRNA of adipogenesis-associated genes was analyzed by qRT-PCR. Relative mRNA of Cebpa, Fabp4, Pparg and Srebp1 was normalized by Gapdh. * p < 0.05 vs. none, # p < 0.05 vs. CO2 or RF2 (Tukey’s honest significant difference post hoc test, analysis of variance). Abbreviations: Undiff—undifferentiated; None—non-treated.