Figure 2.

TM effectively inhibited the production of on nitric oxide (II) (NO) by lipopolysaccharide (LPS)-challenged J774 macrophages. (A) The viability of J774 cells incubated with or without (control) soloxolone methyl (SM) and trioxolone methyl (TM) for 24 h. (B) The effect of triterpenoids on NO production by LPS-activated J774 cells. J774 cells were co-treated by LPS (10 µg/mL) and TM or SM (0.05–1 µM) for 24 h followed by the measurement of the nitrite concentration in a culture medium using the Griess reaction. The results are expressed as the means ± SD of three (A) and two (B) experiments performed in tetra- (A) and pentaplicate (B).