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. 2020 May 16;25(10):2326. doi: 10.3390/molecules25102326

Figure 4.

Figure 4

Effect of EGCG on histone deacetylase activity in a cell-free experimental model. As a positive control, an inhibitor of HDACs - 1 µM of trichostatin A (TSA) was included. HDAC activity was measured by 90 min (left chart) and fluorescence of the probe (7-amino-4-methylcoumarin, AMC) at a 90 min was used for assessment of HDAC activity (right chart), according with the manufacturer’s suggestions. The data are presented as mean ± SD, n = 3; ≈ p < 0.01, # p < 0.01, control vs. treated, ANOVA and post hoc analysis by Tukey’s test.