Figure 6.

Effect of EGCG treatment on methylation status of selected lysines (K) of the histone 3 (H3) in primary HUVECs (A,B) and immortalized HMEC-1 (C,D). Cells were incubated for 16 h with 50, 100 or 200 µM of EGCG. The proteins after acid extraction were used for the immunoblotting analysis. The presented western blots are representative of three independent experiments and the charts visualise densitometry of the chemiluminescence signal calculated vs. loading control and vs. experiment control. The data are presented as mean ± SD; n = 3; * p < 0.05, # p < 0.01, control vs. treated, ANOVA and post hoc analysis by Tukey’s test.