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. 2020 May 14;25(10):2310. doi: 10.3390/molecules25102310

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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HILIC-ESI-FTMS analyses of a plasma lipid extract performed in positive (A) and negative (C) ion mode with full MS acquisition. Extracted ion current chromatograms (XIC) using HILIC-ESI-FTMS AIF scan acquisition under positive (B) and negative (D) ion mode are also reported. XIC traces were obtained using narrow extraction windows centered on selected m/z values corresponding to the following class-diagnostic ions: in positive ion mode (B), m/z 184.073 (PC, sphingomyelin (SM) and LPC, green trace) and m/z 264.270 (sphingolipids, black trace), and in negative ion mode (D), m/z 224.069 (PC and LPC, green trace), m/z 196.038 (PE and LPE, red trace) and m/z 241.012 (PI, blue trace). Putative structures of these ions are shown in the insets.