Table 4.
Results of functional analysis of seven genes selected in silico as targets of miRNA identified as signatures of chronic venous disease.
| Functional Analysis of Upregulated Genes (CDS2, HDAC5, PPP6R2, PRRC2B, TBC1D22A, WNK1) | |
|---|---|
| KEGG, Reactome, GAD and GAD Class | |
| CDS2 | KEGG: Glycerophospholipid metabolism, Phosphatidylinositol signaling system, Metabolic pathways |
| Reactome: Synthesis of PG (Phosphatidylglycerol) | |
| GAD: Type 2 Diabetes|edema|rosiglitazone, Tobacco Use Disorder | |
| GAD Class: pharmacogenomic, chemdependency | |
| HDAC5 | KEGG: Alcoholism, Viral carcinogenesis, |
| Reactome: NOTCH1 Intracellular Domain Regulates Transcription, Constitutive Signaling by NOTCH1 PEST Domain Mutants, Constitutive Signaling by NOTCH1 HD + PEST Domain Mutants | |
| GAD: antidepressant response, Bone Density, Bone mineral density (hip), Bone mineral density (spine), bronchodilator response, Fractures, Bone, Type 2 Diabetes| edema | rosiglitazone | |
| GAD Class: immune, metabolic, pharmacogenomic | |
| PPP6R2 | No information |
| PRRC2B | No information |
| TBC1D22A | GAD: Albumins, Arteries, Attention Deficit Disorder with Hyperactivity, Blood Pressure, Body Mass Index, Body Weight, Breath Tests, Cardiomegaly, Cholesterol, Erythrocyte Count, Fibrinogen, Heart Failure, Heart Rate, Leukocyte Count, longevity, Metabolism, Myocardial Infarction, Parkinson Disease, Resistin, Stroke, Thyrotropin, Tobacco Use Disorder, Waist Circumference, Waist-Hip Ratio |
| GAD Class: aging, cardiovascular, chemdependency, hematological, immune, metabolic, neurological, other, psych | |
| WNK1 | Reactome: Stimuli-sensing channels |
| GAD: Apoplexy|Brain Ischemia|Stroke, blood pressure, arterial, Chronic renal failure|Kidney Failure, Chronic, Essential Hypertension, Hereditary Sensory and Autonomic Neuropathies, HIV Infections|[X]Human immunodeficiency virus disease, hypertension, null, Tobacco Use Disorder, Type 2 Diabetes| edema | rosiglitazone | |
| GAD Class: cardiovascular, chemdependency, infection, neurological, pharmacogenomic, renal, unknown | |
| Gene Ontology terms associated with EASE score <0.1 | |
| GO Biological Process | cellular developmental process, positive regulation of molecular function |
| GO Molecular Function | enzyme binding |
| Functional analysis of downregulated gene (PABPC3) | |
| KEGG, Reactome, GAD and GAD Class | |
| PABPC3 | KEGG: RNA transport, mRNA surveillance pathway, RNA degradation |
| GAD: Body Mass Index, Body Weight, Body Weight Changes, Glomerular Filtration Rate | |
| GAD Class: metabolic, renal | |
| Gene Ontology terms associated with PABPC3 | |
| GO Biological Process | nucleobase-containing compound metabolic process, cellular aromatic compound metabolic process, nitrogen compound metabolic process, metabolic process, cellular process, RNA metabolic process, mRNA metabolic process, cellular nitrogen compound metabolic process, macromolecule metabolic process, cellular metabolic process, primary metabolic process, cellular macromolecule metabolic process, heterocycle metabolic process, organic substance metabolic process, nucleic acid metabolic process, organic cyclic compound metabolic process |
| GO Molecular Function | nucleotide binding, nucleic acid binding, RNA binding, single-stranded RNA binding, binding, poly(A) binding, small molecule binding, poly-purine tract binding, organic cyclic compound binding, nucleoside phosphate binding, heterocyclic compound binding |
| GO Cellular Component | extracellular region, intracellular, cell, cytoplasm, vesicle, membrane-bounded vesicle, organelle, membrane-bounded organelle, extracellular organelle, extracellular region part, intracellular part, cell part, extracellular exosome, extracellular vesicle |
Analysis was carried out with DAVID 6.8 website tool and all associated functional terms of Kyoto Encyclopedia of Genes and Genomes (KEGG), Reactome, Genetic Association Database (GAD), Genetic Association Database Class (GAD Class) categories are presented. Gene Ontology (GO) terms associated with upregulated genes with EASE score (p) < 0.1 are presented. For downregulated gene, all associated GO terms were presented.