Figure 4.

Effects of AIM and TNF-α induced AIM on NF-κB-regulated proteins involved in proliferation, invasion, and angiogenesis. MCF-7 cells were seeded with the seeding density of 5 × 10⁴ cells and pretreated AIM (400 μg/ml) for 1 h, followed by the treatment of TNF-α (10 ng/mL) for 24 h. The control cells were left untreated. The whole cell protein lysate was prepared and 30 μg of proteins were resolved in SDS-Polyacrylamide gels. (A) Western blot analysis of various NF-κB related proteins involved in cancer cell proliferation, invasion, and angiogenesis, (B) Densitometry analysis of the data in western blot analysis by ImageJ software. The values were normalized against β-actin. * p < 0.05, ** p < 0.01 vs. the control group, # p < 0.05, ## p < 0.01 vs. the TNF-α treated group.