Figure 4.

Effects of quercetin and isoquercitrin on VZV lytic-gene expression. HFF cells were inoculated with VZV–pOka-infected HFF cells at an MOI of 0.1, followed by treatment with DMSO, quercetin (Q), and isoquercitrin (Q3G) or 1,2,3,4,6-penta-O-galloyl-ß-D-glucose (PGG) at a concentration of 20 µg/mL. As a control, VZV–pOka-infected HFF cells were treated with ACV (1.2 µg/mL). At 24, 48, and 72 h after infection, total RNA was isolated and reverse-transcribed into cDNA. Relative expression of (A) ORF62 (IE), (B) ORF28 (E) and (C) gB (L) mRNA was assessed via qRT–PCR. For quantification of the relative abundance of viral mRNA between samples, the value of virus-infected cells treated with DMSO was set at 100. Significant differences between samples were determined using Student’s t test (significant at *p < 0.05). Data shown here represent three independent sets of experiments.