Figure 1.

MMP and ADAMTS production in supernatant of synovial cell cultures are displayed. After enzymatic digestion of synovial membrane from patients with advanced knee OA, native synovial cells were cultured for 12 h at 37 °C and 5% CO₂. Enzyme linked immunosorbent assays were then used to analyze enzyme production in culture supernatants. Mean and standard deviation of enzyme concentrations of MMP-1/-3/-9 and ADAMTS-5 are plotted (n = 12). Significant differences are indicated by asterisks: * p < 0.05. In addition, production of MMP-13, ADAMTS-5 and ADAMTS-7 were analyzed, but concentrations were below detection level.