Table 1.
The 30 compounds identified from alfalfa and red clover sprouts by UHPLC-Q-Exactive with structures confirmed by comparison with reference standards.
| Compound Name | R.T. (min) |
Formula | Exact Mass | Error (ppm) |
Adduct Ion (m/z) | MS 2 Fragments (m/z) |
|---|---|---|---|---|---|---|
| Flavonoids (flavan-3-ols, flavone, flavonols, flavonone, flavone glucoside) | ||||||
| Catechin | 7.8 | C15H14O6 | 290.07904 | 1.47 | 289.07176 | 245.08192; 203.07088; 151.03908; 125.02320; 109.02821 |
| Epicatechin | 10.19 | C15H14O6 | 290.07904 | 1.25 | 289.07176 | 245.08192; 203.07088; 151.03908; 109.02821 |
| Quercitin | 16.59 | C15H10O7 | 302.04265 | 0.86 | 301.0354 | 245.04601; 178.99809; 273.04059; 121.02814 |
| Rutin (quercetin3-rutinoside) | 14.20 | C27H30O16 | 610.15338 | 0.5 | 609.14613 | 300.02777; 271.02505; 255.02995; 243.02980; 165.01841;151.00258 |
| Apigenin | 17.54 | C15H10O5 | 270.05282 | 1.18 | 269.04502 | 227.03389; 181.06430; 151, 00194; 149.002266; 117.03271 |
| Kaempferol | 17.06 | C15H10O6 | 286.04774 | 0.57 | 285.04049 | 255.02977; 201.01866; 151.00262; 107.01250; 92.9266 |
| Isorhamnetin | 13.20 | C16H12O7 | 316.0583 | 1.35 | 315.05105 | 300.0271; 227.03508; 163.00369; 151.00264; 107.01190; |
| Naringenin | 19.69 | C15H12O5 | 272.06847 | 0.5 | 271.06122 | 253.05055; 151.00269; 119.04903; 107.01258 |
| Naringin | 14.11 | C27H32O14 | 580.1792 | 2.02 | 579.17185 | 356.99371; 255.02995; 119.04884 |
| Hesperitin | 16.83 | C16H14O6 | 302.07904 | 1.34 | 301.07179 | 283.06204; 267.06650; 252.04286; 151.00266; 125.02319 |
| Pinostrobin | 17.40 | C16H14O4 | 270.08921 | 1.89 | 269.08196 | 254.05864; 210.06839; 177.05495; 148.01559 |
| Pinocembrin | 18.24 | C15H12O4 | 256.07356 | 1.04 | 255.06631 | 239.0713; 237.0557; 227.0713; 179.0349; 147.0451 |
| Chrysin | 17.63 | C15H10O4 | 254.05791 | 1.65 | 253.05066 | 208.96011; 151.03899; 107.04897; 89.04897; 65.03819 |
| Myricetin | 10.42 | C15H10O8 | 318.03757 | 1.37 | 317.03032 | 178.9986; 164.92636; 151.00368; 137.02442; 107.01258 |
| Galangin | 19.98 | C15H10O5 | 270.05282 | 1.48 | 269.04557 | 239.03345; 227.03389; 225.05580; 211.03877; 169.06425 |
| Hyperoside (quercetin 3-galactoside) | 13.98 | C21H20O12 | 464.09548 | 1.03 | 463.08768 | 300.02771; 355.02985; 271.02491; 243.02969; 178.99773; 151.00262 |
| Isoflavone | ||||||
| Genistin | 14.77 | C21H20O10 | 432.10565 | 1.45 | 431.09837 | 311.05637; 269.04590; 271.05133; 181.06580 |
| Genistein | 18.07 | C15H10O5 | 270.05282 | 1.24 | 269.04502 | 159.04420; 133.02835; 201.05527; 181.06546; 107.01257 |
| Daidzin | 11.42 | C21H20O9 | 416.11073 | 1.49 | 415.10348 | 252.0451; 251.0349; 224.0487; 223.0398 |
| Daidzein | 16.50 | C15H10O4 | 254.05791 | 0.87 | 253.05066 | 226.05887; 224.04649; 209.06091; 197.06055; 135.00686; 117.03333 |
| Ononin | 26.14 | C22H22O9 | 430.12638 | 2.06 | 429.11913 | 355.0969; 341.1109; 267.1028; 252.00778 |
| Formononetin | 18.74 | C16H12O4 | 268.07356 | 1.19 | 267.06631 | 252.04298; 223.03986; 195.04466; 132.02049 |
| Glycitein | 16.33 | C16H12O5 | 284.06847 | 1.18 | 283.06122 | 268.0375; 240.0483; 211.03979; 196.05252; 167.02063 |
| Phenolic Acid | ||||||
| Gallic Acid | 1.73 | C7H6O5 | 170.02152 | 0.16 | 169.01427 | 125.02318; 141.01823 |
| Chlorogenic Acid | 8.20 | C16H18O9 | 354.09508 | 0.24 | 353.08783 | 192.05876; 191.05544; 173.04474; 127.03876; 85.02806 |
| Caffeic Acid | 8.71 | C9H8O4 | 180.04226 | 0.37 | 179.03501 | 135.04390; 107.04881 |
| Ferulic Acid | 14.98 | C10H10O4 | 194.05791 | 0.62 | 193.05066 | 178.02635; 149.05974; 134.03615; 106.0424 |
| Ellagic Acid | 14.44 | C14H6O8 | 302.00627 | 1.62 | 300.99899 | 185.02349; 283.98961; 229.01391; 157.01006 |
| Abscinic Acid | 15.73 | C15H20O4 | 264.13616 | 1.42 | 263.12891 | 263.12854; 219.13864; 204.11502; 153.09126; 136.05162 |
| p-coumaric Acid | 10.77 | C9H8O3 | 164.04734 | 0.18 | 163.03954 | 211.0764; 135.00754; 119.0502; 17.0332; 116.0267 |
| Syringic Acid | 15.38 | C9H10O5 | 198.05282 | 0.41 | 197.04555 | 182.02049; 123.00697; 166.99693 |