Figure 2.

Decreased cytosolic active-β-catenin (ABC) and p-β-catenin protein levels in the P7 mouse HP and NC tissues in response to moderate- and high-dose ethanol exposure. The HP and NC cytosolic extracts obtained 4–24 h after the first saline or moderate-dose (A)/high-dose (B) ethanol exposure. The ABC and p-β-catenin protein levels were determined using Western blot analysis. The protein samples were equally loaded, confirmed with Ponceau S staining, and normalized to β-actin. For the 0 h ethanol group, saline was administered. Error bars, SEM (* p < 0.05 vs. the saline [0 h] group, n = 8 pups/group).