Figure 3.

Effect of OOE on apoptosis and cell cycle arrest of BxPC3 cells. BxPC3 cells were seeded in 6-well plates and incubated with 0, 100, and 200 μg/mL OOE for 24 h. (A) Propidium iodide staining analysis of BxPC3 cells was performed by FACS. (B) Annexin V analysis was used to confirm the apoptotic effect of OOE on BxPC3 cells. (C) Data are presented as the mean ± SEM of three separate experiments. (D) The percentages of Annexin-V+/PI− (lower right quadrant) and Annexin-V+/PI+ (upper right quadrant) cells were calculated and shown as a bar graph.* p < 0.5, *** p < 0.001 in comparison to the control.