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. 2020 May 25;25(10):2452. doi: 10.3390/molecules25102452

Figure 2.

Figure 2

In cell western (ICW) analysis of anti-HSV-1 effects of EO (68, CS12). 0.1 m.o.i. HSV-1-infected Vero cells were treated for 24 h with serial dilutions of EO 68 ranging from 0.0312 to 0.5 mg/mL. Untreated HSV-1-infected cells (0 mg/mL EO) were used as comparative control. Fixed cells were incubated with anti-gB primary antibody and then with IRDye 800 CW secondary antibody (green fluorescence) and CellTag 700 Stain (red fluorescence). HSV-1 is a mock infected cell (used as staining control).