Table 1.
Gene ontology analysis of molecular function for (A) increased and (B) decreased differential gene expression, showing canonical groups that are consistently altered across time and uniquely regulated within each behavioral group (CTL, CIE, FSS, and CIE + FSS). The number of genes represent the union of significant genes in that behavioral group and canonical molecular function across time. Results are significantly overrepresented (p < 0.05), uniquely with respect to an individual group and timepoint. CTL: non-dependent alcohol consumption, CIE: chronic intermittent ethanol, and FSS: forced stress swim.
| Group | Name | 000-h | 072-h | 168-h | Number of Genes |
|---|---|---|---|---|---|
| (A) | |||||
| AIR | RNA polymerase II regulatory region sequence-specific DNA binding (GO:0000977) | 5.09E−05 | 4.86E−02 | 1.39E−03 | 89 |
| AIR | kinase binding (GO:0019900) | 3.60E−04 | 1.99E−02 | 3.50E−03 | 84 |
| AIR | RNA polymerase II regulatory region DNA binding (GO:0001012) | 4.78E−04 | 2.16E−02 | 2.48E−03 | 45 |
| AIR | SUMO binding (GO:0032183) | 9.12E−04 | 2.34E−02 | 1.55E−02 | 6 |
| AIR | phosphoprotein phosphatase activity (GO:0004721) | 1.55E−03 | 6.64E−03 | 4.21E−03 | 33 |
| AIR | phosphatidylinositol phosphate kinase activity (GO:0016307) | 2.89E−03 | 1.13E−02 | 4.01E−03 | 7 |
| AIR | protein kinase binding (GO:0019901) | 4.57E−03 | 5.48E−03 | 1.33E−02 | 99 |
| AIR | purine ribonucleoside triphosphate binding (GO:0035639) | 1.51E−02 | 7.31E−03 | 2.75E−02 | 69 |
| AIR | ligand-dependent nuclear receptor transcription coactivator activity (GO:0030374) | 1.70E−02 | 2.42E−02 | 1.04E−02 | 17 |
| AIR | syntaxin-1 binding (GO:0017075) | 1.81E−02 | 1.94E−03 | 2.90E−02 | 6 |
| CIE | phosphatidylinositol binding (GO:0035091) | 4.66E−04 | 3.22E−02 | 9.13E−03 | 30 |
| FSS | histone methyltransferase activity (GO:0042054) | 7.13E−05 | 8.36E−05 | 1.98E−03 | 18 |
| FSS | steroid hormone receptor binding (GO:0035258) | 2.11E−04 | 4.14E−04 | 9.74E−04 | 22 |
| FSS | protein phosphorylated amino acid binding (GO:0045309) | 5.05E−04 | 4.83E−02 | 2.36E−02 | 12 |
| FSS | core promoter binding (GO:0001047) | 4.51E−03 | 1.41E−03 | 2.29E−03 | 24 |
| FSS | protein tyrosine kinase activity (GO:0004713) | 8.19E−03 | 5.03E−03 | 4.90E−02 | 36 |
| FSS | androgen receptor binding (GO:0050681) | 9.99E−03 | 1.28E−04 | 3.80E−03 | 11 |
| FSS | regulatory region DNA binding (GO:0000975) | 2.16E−02 | 1.99E−02 | 2.14E−02 | 44 |
| FSS | methylated histone binding (GO:0035064) | 2.46E−02 | 4.06E−02 | 4.66E−03 | 16 |
| FSS | histone deacetylase binding (GO:0042826) | 2.71E−02 | 3.67E−03 | 2.05E−02 | 19 |
| FSS | cadherin binding (GO:0045296) | 2.90E−02 | 2.44E−02 | 2.62E−02 | 60 |
| CIE + FSS | transmitter-gated ion channel activity (GO:0022824) | 3.11E−02 | 3.23E−02 | 2.54E−04 | 12 |
| (B) | |||||
| AIR | ubiquitin-specific protease binding (GO:1990381) | 4.96E−02 | 3.50E−02 | 2.81E−02 | 6 |
| CIE | GTP binding (GO:0005525) | 7.39E−06 | 9.18E−07 | 1.96E−03 | 43 |
| CIE | purine ribonucleoside binding (GO:0032550) | 1.61E−05 | 3.97E−06 | 9.53E−04 | 45 |
| CIE | guanyl ribonucleotide binding (GO:0032561) | 6.76E−05 | 1.31E−05 | 1.00E−03 | 46 |
| FSS | translation initiation factor activity (GO:0003743) | 2.51E−03 | 4.50E−05 | 4.00E−02 | 11 |
| FSS | translation factor activity, RNA binding (GO:0008135) | 3.24E−03 | 7.82E−05 | 3.21E−02 | 15 |
| CIE + FSS | syntaxin binding (GO:0019905) | 6.20E−03 | 1.99E−04 | 1.40E−02 | 22 |
| CIE + FSS | ubiquitin-protein transferase activity (GO:0004842) | 1.83E−02 | 6.60E−03 | 2.46E−02 | 73 |