KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies and Recombinant Proteins | ||
| Human mAb N5-i5 | Isolated from patient (Guan et al., 2013) | HIV Database: record number 2971 |
| Human mAb C11 | Lewis lab (Guan et al., 2013) | HIV Database: record number 707 |
| Human mAb N5-i5 LALA | Lewis lab | PDB:4H8W;PDB:6CHF |
| Human mAb C11 LALA | Lewis lab | PDB:4FZ8;PDB:6CJC |
| Human mAb N12-i2 LALA | Lewis lab | PDB:3QEG; 6CJX |
| Human mAb Synagis (Palivizumab) | Medimmune | 60574-4114-1 |
| Human Recombinant FcγR receptor I/CD64 | R&D systems | Cat# 1257-FC-050 |
| Human Recombinant FcγR receptor IIA/CD32a | R&D systems | Cat# 1330-CD-050 |
| Human Recombinant FcγR receptor IIB/CD32b | R&D systems | Cat# 1875-CD-050 |
| Human Recombinant FcγR receptor IIIA/CD16a | R&D systems | Cat# 4325-FC-050 |
| Horseradish peroxidase conjugated Fab’2 goat anti-human IgG (H+L) | KPL | Cat# 214-1006 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| RPMI 1640 medium | ThermoFisher | Cat# 11875085 |
| fetal bovine serum (FBS) | Gibco | Cat# 16140-071 |
| HEPES buffer | Sigma | Cat# H0887 |
| Sodium Pyruvate | Sigma | Cat# S8636 |
| β-Mercaptoethanol | Gibco | Cat# 21985-023 |
| Gentimicin | Gibco | Cat# 15750-060 |
| G418 Sulfate Solution | Gemini Bio - Products | Cat# 400-113 |
| FreeStyle 293 Expression Medium | ThermoFisher | Cat# 12338018 |
| HIV-1 Ba-L gp120 | Lewis lab (Guan et al., 2013) | N/A |
| Full length single chain protein | (Fouts et al., 2000) | FLSC |
| Amicon Pro Affinity Concentration Kit Ni-NTA | EMD Millipore | Cat# ACK5010NT |
| Amicon ultra 0.5ML - 10KDa cutoff | EMD Millipore | Cat# UFC501024 |
| Pierce™ Nickel Coated Plates, Clear, 96-Well | Thermo Fisher Scientific | Cat # 15442 |
| TMB substrate | Southern Biotech | Cat# 0410-01L |
| HiTrap protein A column | GE Healthcare | Cat# GE17-0402-01 |
| Immobilized papain | Pierce | Cat# 20341 |
| Protein A column | GE Healthcare | Cat# GE17-0402-01 |
| Superdex 200 16/60 column | GE Healthcare | Cat# 28989335 |
| Superdex 200 10/300 column | GE Healthcare | Cat# 17517501 |
| Waters Enzymate BEH pepsin column | Waters | Cat # 186007233 |
| Acquity UPLC BEH C18 peptide trap | Waters | Cat # 186003975 |
| Acquity UPLC BEH C18 column | Waters | Cat # 186002346 |
| Critical Commercial Assays | ||
| Alexa Fluor 647 Antibody Labeling Kit | Thermo Fisher Scientific | Cat# A20186 |
| SNAP-Surface Alexa Fluor 647 | New England BioLabs | Cat# S9136S |
| Deposited Data | ||
| N5-i5 Fab | (Acharya et al., 2014) | Protein Data Bank ID 4H8W |
| IGG1 FC FRAGMENT-FC-GAMMA RECEPTOR III COMPLEX | (Sondermann et al., 2000) | Protein Data Bank ID 1E4K |
| Fucosylated Fc Fragment from Human Immunoglobulin G1 | (Matsumiya et al. 2007) | Protein Data Bank ID 3AVE |
| human IgG1 Fc fragment Heterodimer | (Strop et al. 2012) | Protein Data Bank ID 4DZ8 |
| human IgG1 Fc-fragment,high salt condition | (Krapp et al. 2003) | Protein Data Bank ID 1H3Y |
| HUMAN IGG1 FC-FRAGMENT,GLYCOFORM (G2F)2,SG P212121 | (Krapp et al. 2003) | Protein Data Bank ID 1H3V |
| N5-i5 Fc-LALA structure | This work | Protein Data Bank ID 6CHF |
| C11 Fc-LALA structure | This work | Protein Data Bank ID 6CJC |
| N12-i2 Fc-LALA structure | This work | Protein Data Bank ID 6CJX |
| Experimental Models: Cell Lines | ||
| EGFP-CEM-NKr-CCR5-SNAP cells | (Orlandi et al., 2016) | N/A |
| HEK-293Fs | Thermo Fisher Scientific | Cat # R79007 |
| Recombinant DNA | ||
| N5-i5 LALA Heavy Chain expression plasmid | Lewis lab | N/A |
| C11 LALA Heavy Chain expression plasmid | Lewis lab | N/A |
| N12-i2 LALA Heavy Chain expression plasmid | Lewis lab | N/A |
| Software and Algorithms | ||
| FlowJo software | Tree Star, Inc., San Carlos, Calif. | https://www.flowjo.com |
| Symphotime software | PicoQuant | https://www.picoquant.com/ |
| ORIGIN 5.0 software | Microcal Inc. | https://www.originlab.com |
| PyMol Molecular Graphic suite | DeLano Scientific, San Carlos, CA, USA | https://pymol.org |
| MolProbity | (Chen et al., 2010) | http://molprobity.biochem.duke.edu/ |
| ProteinLynx 649 Global Server 2.5.1 (PLGS) | Waters | www.waters.com |
| DynamX | Waters | www.waters.com |
| GraphPad Prism | GraphPad Software, San Diego, CA, USA | https://www.graphpad.com |
| HKL2000 | (Otwinowski et al., 1997) | https://hkl-xray.com |
| Phaser | (McCoy, 2007) | https://www.ccp4.ac.uk/ |
| CCP4 suite | No authors | https://www.ccp4.ac.uk/ |
| Refmac | (Murshudov et al., 1997) | https://www.ccp4.ac.uk/ |
| Phenix | (Adams et al., 2002) | https://www.phenix-online.org/ |
| COOT | (Emsley and Cowtan, 2004) | https://www2.mrc-lmb.cam.ac.uk |
| Dali server | (Holm, 2019) | http://ekhidna.biocenter.helsinki.fi/dali_server |
| lsqkab | (Kabsch et al., 1976) | https://www.ccp4.ac.uk/ |
| PISA | (Krissinel and Henrick, 2007) | https://www.ebi.ac.uk/pdbe/pisa/ |
| PIC | (Tina et al., 2007) | http://pic.mbu.iisc.ernet.in/ |