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. 2020 Jun 2;2020:1657896. doi: 10.1155/2020/1657896

Figure 4.

Figure 4

Inhibition of autophagy promotes the antiproliferative effect of JK184 in BCa cells. (a-b) JK184 inhibited the growth of BCa cells independent of apoptosis and ferroptosis. Cells were treated with DMSO, Z-VAD-FMK (20 μM), or ferrostatin-1 (1 μM) in the presence or absence of JK184 (4 nM) for 24 hours. (c–f) Cells were treated with DMSO, chloroquine (CQ), or 3-methyladenine (3-MA) in the presence or absence of JK184 (4 nM) for 24 hours. (g) Cells were transfected with shRNA against Atg5 or Beclin 1 or control (50 nM) for 48 h and then treated with JK184 at 4 nm for another 24 hours. (h) Cell viability of BCa cells transferred with shATG5 or shBeclin1 followed by treatment with an indicated concentration of JK184. (i-j) Colony formation assay of BCa cells transferred with shATG5 or shBeclin1 followed by treatment with 4 nM JK184. Representative images (left) and survival fraction were shown (right). ns, P > 0.05; ∗∗, P < 0.01.