Figure 5.

JK184 induces autophagy by inhibiting the Akt/mTOR pathway. (a) Immunoblot analysis of phosphorylation of Akt, mTOR, p70S6K, and 4EBP1 in cells treated with the indicated concentrations of JK184 for 24 hours. The expression levels of total Akt, mTOR, p70s6k, and 4EBP1 were used as internal controls. (b) Densitometry quantification of the band intensities in A (DMSO- and 4 nM JK184-treated groups) was carried out using ImageJ software and is presented by normalizing the values of the DMSO group to 1, relative to the corresponding β-actin.