Figure 1.

Topological phylogenetic tree representation of 226 representatives of the HDAC protein family. The longest RefSeq protein isoform was selected for each separate gene locus. Multiple sequence alignment was performed using the MUSCLE algorithm [32]. Evolutionary distances were computed as the number of amino acid substitutions per site using the Poisson correction method [33]. The implementation of these algorithms and the visualization were achieved through MEGA X [34]. All 226 sequences used for the generation of this figure are available as Supplementary File S2, in FASTA format. Coloring of branches indicate the putative HDAC class: red for Class I, magenta for Class IIa, green for Class IIb, and cyan for Class IV. Colored areas delimit clades associated to each one of the 11 human HDACs.