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Biochemical evaluation of scaffolds over 5 weeks of culture. (A) Cell viability assessed through CellTiter-Blue assay. The fluorescence is normalized to the electrospun control scaffolds at 24 h. (B) DNA quantification measured using PicoGreen assay. (C) DNA normalized to cell viability. Error bars = standard error mean, n = 4. Statistical significance denoted by *p < 0.05, **p < 0.01; one-way ANOVA with Tukey post hoc test. P/S: phase-separated control; ESP: electrospun control.
