Figure 6.

ZC4H2 regulates Gli signaling, subcellular distribution, and ubiquitination in vivo. (A) Real-time PCR results showing the relative Gli1, Ptch1, and Hhip1 expression in wild-type (WT) or ZC4H2 knockout (KO) neural stem cells. ** P < 0.01 (Student’s t-test). (B) Western blotting assays showing the subcellular distribution of endogenous Glis in ZC4H2 WT or KO neural stem cells. (C) The levels of K63-ubiquitinated Glis in ZC4H2 WT or KO MEF cells. The cell lysates were immunoprecipitated with K63-ubiquitin chain-specific antibody or control IgG before blotted using Gli antibodies. The relative densitometrical statistics are shown below.