TABLE 1.
Characteristics of linezolid-resistance plasmids in 26 E. faecalis isolates carrying optrA.
| Isolate ID | Plasmid size (kb) | Rep-familya | Prototypea | Transferred resistance genea | MICs of linezolid (mg/L)b | MLSTc |
| P10748 | ∼54 | 9 | pCF10 | optrA, tetL, ermB, lnuB, fexA | 8 | ST480 |
| EF-3015 | ∼150 | 6, 9 | pS86, pCF10 | optrA, tetL, ermB, lnuB fexA | 8 | ST828 |
| EF-7013 | ∼60 | 9 | pCF10 | optrA, tetL, ermB, lnuB, fexA | 8 | ST16 |
| EF-8194 | ∼80 | 9 | pCF10 | optrA, tetL ermB, lnuB fexA | 8 | ST16 |
| EF-9289 | ∼80 | 1, 7, 9 | pIP501, pUSA02, pAD1 | optrA, tetL, ermB, fexA | 8 | ST826 |
| EF-3139 | ∼100 | 1, 9 | pIP501, pCF10 | optrA, tetL, tetM, ermB, lnuB fexA | 16 | ST386 |
| EF-5136 | ∼80 | 9 | pCF10 | optrA, tetL, tetM, ermB, lnuB fexA | 8 | ST16 |
| EF-2084 | ∼80 | 1, 9 | pIP501, pCF10 | optrA, tetL, tetM, ermB, lnuB, fexA | 8 | ST480 |
| EF-0132 | ∼80 | 1, 9 | pIP501, pCF10 | optrA, tetL ermB, lnuB, fexA | 16 | ST585 |
| EF-4003 | ∼55, ∼350d | 7 | pUSA02 | optrA, tetL, tetM, ermB, fexA | 16 | ST16 |
| EF-8042 | ∼30, ∼200d | 1, 9 | pIP501, pCF10 | optrA, tetL, ermB, fexA | 8 | ST69 |
| EF-0074 | ∼65 | 7, 9 | pUSA02,pAD1 | optrA, tetL, ermB, lnuB, fexA | 8 | ST826 |
| EF-6165 | ∼80 | 1 | pIP501 | optrA, tetL, tetM, ermB, lnuB, fexA | 8 | ST823 |
| EF-7094 | ∼40, ∼350d | 9 | pCF10 | optrA, tetL, tetM, ermB, lnuB, fexA | 8 | ST632 |
| EF-1127 | ∼55 | 1, 9 | pIP501, pCF10 | optrA, tetL, tetM, ermB, lnuB, fexA | 8 | ST824 |
| EF-8137 | ∼80 | 1, 7, 9 | pIP501, pUSA02, pCF10 | optrA, tetL, tetM, ermB, lnuB, fexA | 8 | ST116 |
| EF-8282 | ∼80 | 6,9 | pS86, pCF10 | optrA, tetL, ermB, lnuB | 16 | ST330 |
| EF-4245 | ∼80 | 9 | pCF10 | optrA, tetL, ermB, lnuB, fexA | 8 | ST823 |
| EF-1080 | ∼40 | 9 | pCF10 | optrA, tetL, tetM, ermB, lnuB, fexA | 8 | ST69 |
| EF-2216 | ∼80 | 1,9 | pIP501,pCF10 | optrA, tetL, tetM, ermB, lnuB, fexA | 8 | ST376 |
| EF-6166 | ∼80 | NA | NA | optrA, tetL, ermB, lnuB, fexA | 16 | ST16 |
| EF-5015 | ∼80 | NA | NA | optrA, tetL, tetM, ermB, lnuB, fexA | 8 | ST480 |
| EF-2021 | ∼90 | NA | NA | optrA, tetL, ermB, lnuB fexA | 16 | ST714 |
| EF-8014 | ∼55 | NT | NT | NT | 8 | ST632 |
| EF-3186 | ∼30 | NT | NT | NT | 8 | ST69 |
| EF-1090 | ∼55 | NT | NT | NT | 8 | ST585 |
aThese results are consistent between transconjugants and original clinical isolates. bAll clinical isolates considered low-level resistance to linezolid (Hua et al., 2019). MIC values for other drugs are listed in Supplementary Table S1. cMulti-locus sequence types determined for original isolates in our previous study (Hua et al., 2019). dTwo plasmids with different sizes. NA, not available in plasmid typing with 19 known rep families. NT, not tested due to negative results in conjugation experiment.