Table 2.

In vitro models Used to Assess Steatohepatitis

Species	Cell Type	Drugs Tested	Endpoints Used	Insulin concentration	Glucose concentration	References
Mouse	Primary Hepatocytes	Amiodarone, Tamoxifen	Microarrays, Differential in gel electrophoresis, Bioenergetics	3.5 μmol/L	4500 mg/L	Summeren 2013, Le et al 2014
	Precision Cut Liver Slices	Amiodarone, Valproic Acid, and Tetracycline		–	2000 mg/L	Szalowska et al 2014
Rat	Primary Hepatocytes	Diethylaminoethoxyhexestrol, amiodarone, perhexiline, valproate, tetracycline, tamoxifen	ATP, β-oxidation, triglycerides, lipid peroxidation, mitochondrial respiration dysfunction, oxidative stress	0.7 μmol/L	2000 mg/L	Deschamps et al 1994, Berson et al 1998, Tong et al 2005 and Choi 2015, Lelliott at al 2005.
	NT H35 hepatoma cell line	Oleic acid, linoleic acid	Oil red O, triglycerides, Reactive Oxygen Species, Mitochondrial Membrane Potential	3 μmol/L	–	Janorkar 2009
Dog	Primary Hepatocytes	Tetracycline	Oil red O, triglyceride content Nile red, β-oxidation	0.05 μmol/L	10000mg/L	David E. Amacher and Barbara-Anne Martin 1997
Human	Primary Hepatocytes	76 nuclear receptor ligands	Lipid accumulation, mRNA	–	–	Moya et al 2010
	HepG2 cancer cell line	Oleic acid, Amiodarone, Doxycycline, Tetracycline, Valproate, Tamoxifen	Mitochondrial respiration dysfunction, oxidative stress, Oil red O, mRNA	–	–	Donato 2009, Choi 2015, Kamalian 2015, Zhao et al 2014.
	Huh7 cell line	u-PA, valproate	Triglyceride, lipid droplets	–	–	Paland 2014, Elphick 2012
	HepaRG Cells	Amiodarone and Tetracycline	Oil red O	0.86 μmol/L	2000 mg/L	Antherieu 2011
	iPSC derived hepatocytes	Amiodarone, Propranolol	Mitochondrial potential, LipidTOX (phospholipids and neutral lipids)	–	–	Sirenko et al 2014
	hSKP-HPCs postnatal skin precursor derived Hepatocytes	Sodium Valproate	Microarrays, LipidTox stains, transmission electron microscopy	1.0 μmol/L	4500 mg/L	Rodrigues 2015