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Binding of peptides 1 and 2 to Keap1 as monitored by fluorescence polarization (FP). (a) Binding of fluorescein-labeled peptides (20 nM) as a function of Keap1 concentration; (b) Competition for binding to Keap1 (40 nM) between fluorescein-labeled peptide 2 (20 nM) and increasing concentrations of unlabeled peptide 1 or 2. Data shown represent the mean ± SD of three independent experiments.
