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. 2020 Jun 4;131(4):993–999. doi: 10.1213/ANE.0000000000005044

Figure 1.

Figure 1.

Flow cytometric analysis of HLA-DR expression in COVID-19 patients. Gating strategy: key steps in measurement of monocytic HLA-DR expression using standardized assessment. Beads are gated on their SSC and FSC characteristics (not shown), and PE fluorescence is plotted (A). Patient’s monocytes are gated by CD14-binding and SSC properties (B), mHLA-DR expression is plotted against CD14 to calculate the median HLA-DR expression (C). CD14 indicates cluster of differentiation (14); COVID-19, coronavirus disease 2019; FSC, forward scatter; HLA-DR, human leukocyte antigen-DR; mHLA-DR, monocytic human leukocyte antigen-DR; PE, phycoerythrin; SSC, side scatter.