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NAC1 expression modulates BCL6 transcription. (A) OSE4 cells were transfected with NAC1-V5 constructs. BCL6 expression was assessed by qPCR 48 h post-transfection. NAC1-targeting siRNA was transfected into OVCAR-5 (B), OVCAR-3 (C), SKOV3 (D), and SKOV3TR (E) cells 48h prior to analysis of BCL6 expression by qPCR. (F) BCL6 mRNA expression was assessed in Nacc1-/- mouse spleen tissue. *P<0.05, **P<0.01.
