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. 2020 May 29;12(10):9031–9040. doi: 10.18632/aging.103164

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Copyright © 2020 Nashine and Kinney.

This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY) 3.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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(A) Upper and lower panels show Representative Incucyte live-cell images of untreated and Citicoline-treated AMD cells ,respectively, in phase-contrast (first column), stained with NucLight Red (second column), stained with Caspase-3/7 Green (third column), overlap i.e., Caspase-3/7 + NucLight (fourth column), and Merge i.e., Phase-contrast + Caspase-3/7 + NucLight (fifth column). Scale bar = 400 μM.(B) Quantitation of Caspase-3/7 overlap/ Red object count at the 48 h time point. (C) Quantitation of Caspase-3/7 overlap/ Red object count at the 72 h time point.