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. 2020 May 28;12(10):9793–9806. doi: 10.18632/aging.103244

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Copyright © 2020 Wang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Knockdown of hsa_circ_0003141 inhibits tumorigenesis of Huh-7 subcutaneous xenografts in vivo. Mice were s.c. implanted with hsa_circ_0003141-shRNA1-transfected Huh-7 cells. (A) Xenograft tumor volume monitored weekly. (B, C) Xenografts tumors were photographed and calculated. (D) Expression of UBAP2 and cleaved caspase 3 in tumor tissues analyzed by western blotting. (E, F) The relative expression of UBAP2 and cleaved caspase 3 in tumor tissues normalized to β-actin. **P < 0.01, compared with the shRNA-NC group. ^##P < 0.01, compared with the circRNA_shRNA1 group.