Figure 1.

Loss of function mutations in dACOX1 in flies cause lethality

(A) Schematic representation of the molecular lesion in dACOX1, the dACOX1 deletion (dACOX1^ywg), and the insertional mutation (dACOX1^T2A). (B) Summary of complementation tests. GR: Genomic Rescue construct. The numbers indicate the # of observed flies versus # of expected flies (C) Western blot of dACOX1 with protein lysates from 3rd instar larvae of dACOX1^T2A;GR (control), dACOX1^ywg and dACOX1^T2A showing diminished dACOX1 level with both mutant alleles. Mean ± s.e.m. ***p< 0.001. (D) Larval growth curves of control, dACOX1^ywg and dACOX1^T2A animals. (E) dACOX1 is expressed in glia of larval CNS. The expression of dACOX1^T2A>nls-mCherry (magenta) colocalized with anti-Repo (green), marking the glia nuclei, in larval CNS. Scale bar: 15μm. (F) dACOX1 is expressed in most glia of adult flies. The expression of dACOX1^T2A>UAS-nls-mCherry (magenta) colocalized with anti-Repo (green), marking the glia nuclei, in the adult fly brain. Scale bar: 5μm